• Effects of mTORC1 inhibition on proteasome activity and levels

    Characterization of purified 26S proteasome after treatment with Torin1. (A, B) Effect of mTORC1 inhibitor Torin1 on proteasomes. HEK293 cells were treated with 250 nM Torin1 for indicated time periods. Whole-cell lysates (A) and purified proteasomes (B) were collected and separated on denaturing SDS-PAGE. (C) Comparison of phosphorylation of purified proteasomes using pan-phosphorylation antibodies (61-8300, Invitrogen, 1.0 μg/ml). (D, E) Effect of Torin1 treatment on proteasome activity. Purified proteasomes (2 ug) were separated on non-denaturing (native) PAGE, followed by in-gel suc-LLVY-AMC hydrolysis or immunoblotting against the 20S subunit, PSMA4, to visualize proteasomes (D). Protein samples (10 μg) were subjected to suc-LLVY-AMC (12.5 μM) hydrolysis to estimate proteasome activity (E).
  • Roles of heterogenous hepatic macrophages in the progression of liver diseases

    Roles of heterogenous liver macrophages during the progression of liver injury. Under the pathologic process of liver injury, the resident embryonic-derived Kupffer cells (EmKCs) located inside the sinusoidal endothelium recognize microbial pathogen-associated molecular patterns (PAMPs) and damaged cell-released damage-associated molecular pattern (DAMPs) via PRRs and inflammasome. The activated EmKCs release inflammatory cytokines and CCL2 chemokine to recruit circulating monocytes. They develop into inflammatory Ly6Cpos monocyte-derived macrophages (MoMFs) or anti-inflammatory Ly6Cneg MoMFs to promote or suppress fibrotic activation of hepatic stellate cells (HSCs). MoMFs can fully differentiate toward monocyte-derived KCs (MoKCs) by activation of LXRα and ID3 transcription factors. TREM2 and CD9 positive lipid-associated macrophages (LAMs) are derived from MoKCs and their roles in the development of liver injury has not been identified yet. The stimulation, differentiation, and conversion of hepatic macrophages are indicated as shown colored arrows.

BMB Reports 2022; 55(4): 161~204
Invited Mini Reviews
Effects of mTORC1 inhibition on proteasome activity and levels
Seo Hyeong Park , Won Hoon Choi & Min Jae Lee
BMB Reports 2022; 55(4): 161-165  https://doi.org/10.5483/BMBRep.2022.55.4.032
Roles of heterogenous hepatic macrophages in the progression of liver diseases
Kyeong-Jin Lee, Mi-Yeon Kim & Yong-Hyun Han
BMB Reports 2022; 55(4): 166-174  https://doi.org/10.5483/BMBRep.2022.55.4.022
Articles
Application of periostin peptide-decorated self-assembled protein cage nanoparticles for therapeutic angiogenesis
Ba Reun Kim, Jung Won Yoon, Hyukjun Choi, Dasol Kim, Sebyung Kang & Jae Ho Kim
BMB Reports 2022; 55(4): 175-180  https://doi.org/10.5483/BMBRep.2022.55.4.137
Reactive microglia and mitochondrial unfolded protein response following ventriculomegaly and behavior defects in kaolin-induced hydrocephalus
Jiebo Zhu , Min Joung Lee, Hee Jin Chang, Xianshu Ju, Jianchen Cui, Yu Lim Lee, Dahyun Go, Woosuk Chung, Eungseok Oh & Jun Young Heo
BMB Reports 2022; 55(4): 181-186  https://doi.org/10.5483/BMBRep.2022.55.4.126
Exercise-induced beige adipogenesis of iWAT in Cidea reporter mice
Jin Kyung Kim, Hye Sun Go, Sol Pin Kim, Il Yong Kim, Yun Hee Lee , Seung Hyun Oh , Ho Lee & Je Kyung Seong
BMB Reports 2022; 55(4): 187-191  https://doi.org/10.5483/BMBRep.2022.55.4.134
Casein kinase 2 promotes the TGF-β-induced activation of α-tubulin acetyltransferase 1 in fibroblasts cultured on a soft matrix
Eunae You, Jangho Jeong, Jieun Lee, Seula Keum, Ye Eun Hwang, Jee-Hye Choi & Sangmyung Rhee
BMB Reports 2022; 55(4): 192-197  https://doi.org/10.5483/BMBRep.2022.55.4.021
SOCS1 counteracts ROS-mediated survival signals and promotes apoptosis by modulating cell cycle to increase radiosensitivity of colorectal cancer cells
Ji-Yoon Ryu, Jiyoung Oh, Su-Min Kim, Won-Gi Kim, Hana Jeong, Shin-Ae Ahn, Seol-Hee Kim, Ji-Young Jang, Byong Chul Yoo, Chul Woo Kim & Choong-Eun Lee
BMB Reports 2022; 55(4): 198-203  https://doi.org/10.5483/BMBRep.2022.55.4.191
Erratum
Erratum to: Stem cell-derived extracellular vesicle therapy for acute brain insults and neurodegenerative diseases
Oh Young Bang & Ji-Eun Kim
BMB Reports 2022; 55(4): 204-204  

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April 2022
Volume 55
Issue 4

2020 SCI Impact Factor 4.778

BIOCHEMISTRY & MOLECULAR BIOLOGY 98/298

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