BMB Rep. 2015; 48(12): 702-707  
Stanniocalcin 2 enhances mesenchymal stem cell survival by suppressing oxidative stress
Pyung-Hwan Kim1,2, Sang-Su Na2, Bomnaerin Lee2, Joo-Hyun Kim2 & Je-Yoel Cho2,*
1Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, Daejeon 35365, Korea, 2Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
Correspondence to: Tel: +82-2-880-1268; Fax: +82-2-886-1268; E-mail: jeycho@snu.ac.kr
Received: July 23, 2015; Revised: August 13, 2015; Accepted: September 25, 2015; Published online: December 31, 2015.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

ABSTRACT
To overcome the disadvantages of stem cell-based cell therapy like low cell survival at the disease site, we used stanniocalcin 2 (STC2), a family of secreted glycoprotein hormones that function to inhibit apoptosis and oxidative damage and to induce proliferation. STC2 gene was transfected into two kinds of stem cells to prolong cell survival and protect the cells from the damage by oxidative stress. The stem cells expressing STC2 exhibited increased cell viability and improved cell survival as well as elevated expression of the pluripotency and self-renewal markers (Oct4 and Nanog) under sub-lethal oxidative conditions. Up-regulation of CDK2 and CDK4 and down-regulation of cell cycle inhibitors p16 and p21 were observed after the delivery of STC2. Furthermore, STC2 transduction activated pAKT and pERK 1/2 signal pathways. Taken together, the STC2 can be used to enhance cell survival and maintain long-term stemness in therapeutic use of stem cells.
Keywords: Mesenchymal stem cells, Oxidative stress, Stanniocalcin- 2, Stem cell-based therapy

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