BMB Rep. 2014; 47(2): 98-103  
Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway
Hyeong-Seon Lee1,2,#, Gyeong-Seon Lee1,2,#, Seon-Hee Kim1,2, Hyun-Kyung Kim4, Dong-Hee Suk1,2,3 & Dong-Seok Lee1,2,*
Departments of 1Smart Foods and Drugs, 2Biomedical Laboratory Science, Inje University, Gimhae 621-749, 3Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan 614-735, 4Department of Biomedical Laboratory Science, College of Natural Science, Gimcheon University, Gimcheon 740-704, Korea
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Received: April 19, 2013; Revised: May 14, 2013; Accepted: June 13, 2013; Published online: February 28, 2014.
© Korean Society for Biochemistry and Molecular Biology. All rights reserved.

Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.
Keywords: Anti-oxidizing effect, HO-1, MAPK, Nrf2/Keap1, Orostachys japonicus

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