Abnormal kidney morphology in PDIA6-knockdown mice. (A) The sgRNA sequences targeting Pdia6 are highlighted in blue, and PAM sequences in red. The Pdia6+/− mice are characterized by a deletion of 58 bp in the exon 4 region. This panel shows a representative PCR genotyping for WT and Pdia6+/− mice. (B) Pdia6 depletion enhances ferroptosis in the kidneys of Pdia6+/− mice, assessed by western blotting using specific antibodies. (C) Kidney tissues from WT and Pdia6+/− mice were immunostained with ARL13B antibody (green) and acetylated tubulin (red), and visualized using confocal microscopy. Scale bar: 10 μm. (D) The quantification of ciliated cells and cilium length are presented graphically. Measurements were obtained from approximately 200 cells per group. (E) H&E staining reveals morphological abnormalities in the glomeruli of Pdia6+/− mice, with arrows highlighting the glomeruli. Scale bar: 10 μm. (F) Perls Prussian blue staining shows iron deposition in kidney sections, particularly in the areas of the inner stripe of the outer membrane, as indicated by arrows. Scale bar: 100 μm. (G) Schematic of the PDIA6-knockdown lead to ferroptosis. Data are expressed as the mean ± SD. Statistical significance was assessed using one-way ANOVA test, ***P < 0.001.
