Modulation of primary cilia dysregulation by inhibiting ER Stress in PDIA6-depleted cells. (A-C) Following transfection with siRNA, cells were treated with or without tauroursodeoxycholic acid (TUDCA, 10 μM) for 24 h. (A) Western blot analysis using specific antibodies. (B) Primary cilia were immunostained, and imaging was conducted by fluorescence microscopy. (C) The numbers of ciliated cells and cilia lengths are displayed graphically. Measurements were obtained from approximately 200 cells per group. Data are presented as the mean ± SD. Statistical significance was assessed using one-way ANOVA test, ns: not significant, ***P < 0.001. Scale bar: 10 μm.
