Reversal of primary ciliogenesis dysregulation in PDIA6-depleted HK-2 cells by ferroptosis inhibition. (A-C) Following transfection with siRNA, cells were treated with ferroptosis inhibitors. ferrostatin-1 (Fer-1, 1 μM) and liproxstatin-1 (Lip-1, 1 μM) for 24 h. (A) Primary cilia were immunostained, and imaging was conducted by fluorescence microscopy. (B) The numbers of ciliated cells and cilia lengths are represented graphically. Measurements were taken from approximately 200 cells per group. (C) Western blot analysis using specific antibodies. Data are presented as the mean ± SD. Statistical significance was assessed using one-way ANOVA test, ***P < 0.001. Scale bar: 10 μm.
