Depletion of PDIA6 impairs primary cilia and sensitizes cells to ferroptosis in HK-2 cells. (A) Primary cilia were immunostained, and imaging was conducted by fluorescence microscopy. (B) The number of ciliated cells and cilia lengths are graphically represented. (C) Western blot analysis using antibodies targeting ferroptosis-related protein. (D) The fluorescence of DCFH-DA staining represents ROS. The relative intensity of DCF is graphically represented. (E) The green fluorescence of C11-BODIPY represents lipid peroxidation. The relative intensity of DCF is graphically represented. (F) Western blot analysis confirmed lipid peroxidation. (G) Intracellular Fe2+ levels were detected using FerroOrange, with the relative intensity of FerroOrange also being displayed graphically. Data are expressed as the mean ± SD. Statistical significance was determined using the Mann-Whitney and one-way ANOVA test, **P < 0.01, ***P < 0.001. Scale bar: 10 μm.
