Cervical cancer patients with high MFN2 expression showed a poor prognosis. (A) β-catenin abundance (left panel) and TCF/LEF reporter (TOPFlash) activity (right panel) were significantly lowered in SiHa cells with MFN2-knockdown compared to that in Scr-shRNA control cells. The results were analyzed using ANOVA followed by Dunnett’s post-hoc test (**P < 0.01, ***P < 0.001 vs. Scr-shRNA). All experiments were performed in triplicate. In silico data from cervical cancer patients were correlated with MFN2 expression. (B) Comparison of mutation counts between groups with high and low MFN2 expression. (C) The overall survival curve for cervical cancer patients showing high and low MFN2 expression was obtained using the cBioPortal (https://www.cbioportal.org/).
