rhHAPLN1 suppresses PDGFRβ, FAK and Ras/MAPK/STAT3 pathways activated by PDGF-BB in HASMCs. Western blots conducted on HASMCs after treatment with rhHAPLN1 (concentrations indicated on figure) for 48 h or 10 ng/ml PDGF-BB for 5 min. The following protein levels were measured: (A) p-PDGFRβ/PDGFRβ, (B) p-FAK/FAK, (C) p-JNK/JNK, p-p38/p38, and p-ERK/ERK, p-STAT3/STAT3, (D) Ras/p-c-Raf/c-Raf. P-values indicated by # were calculated relative to untreated cells, whereas P-values indicated by * were calculated relative to PDGF-BB treated cells. * or # indicates P < 0.05, ** or ## indicates P < 0.001, *** or ### indicates P < 0.0005, and **** or #### indicates P < 0.0001 (N = 3 per group).
