rhHAPLN1 inhibits PDGF-BB elevated HASMCs migration, and secretion of inflammatory factors. (A) Inverted microscopy images of confluent HASMCs 8 hours after wounding. The percentage of area covered by migrated cells over time was calculated using ImageJ, and these results are presented in the panel on the right. Scale bar: 100 μm. (B) The protein expression level of MMP-9 in HASMCs after treatment with rhHAPLN1 (concentrations indicated on figure) or 10 ng/ml PDGF-BB for 48 h. (C) qRT-PCR results of mRNA expression levels of inflammatory factors, TNF-a, IL-1β, and IL-8 in HASMCs after treatment with 20 ng/ml rhHAPLN1 or 10 ng/ml PDGF-BB for 48 h. (D) The protein expression levels of p-AKT/AKT, p-NF-κB/NF-κB was determined by Western blot analysis. P-values indicated by # were calculated relative to untreated cells, whereas P-values indicated by * were calculated relative to PDGF-BB treated cells. * or # indicates P < 0.05, ** or ## indicates P < 0.001, *** or ### indicates P < 0.0005, and **** or #### indicates P < 0.0001 (N = 3 per group).