Inhibition of USP14 activity suppressed 5-FU resistance in gastric cancer cells. (A) Immunoblotting analysis for assessing the levels of USP14 and PARP levels in SNU620 cells after treatment with DMSO, 5-FU (100 μM), and IU1 (100 μM) and co-treatment with 5-FU/IU1. (B) Effects of treatment with 5-FU (100 μM) and IU1 (100 μM) and co-treatment with 5-FU/IU1 on the viability of SNU620 cells. (n = 5, *P < 0.05, **P < 0.01). (C) Immunoblotting analysis for assessing the levels of USP14 and PARP after treatment with DMSO, 5-FU (100 μM), and IU1 (100 μM) and co-treatment with 5-FU/IU1 in 5-FU-resistance SNU620 cells. (D) Effects of treatment with 5-FU (100 μM) and IU1 (100 μM) and co-treatment with 5-FU/IU1 on the viability of 5-FU-resistant SNU620 cells (n = 5, **P < 0.01, ***P < 0.001).
