USP14 expression level in gastric cancer. (A) Representative immunohistochemistry (IHC) for USP14 in paired tumor and adjacent non-tumor tissues. Scale bar, 50 μm. N: Non-tumor tissue, T: Tumor tissue. (B) Pie chart representing the number of patients stratified based on the expression level of USP14 protein. (C) mRNA expression of USP14 in 76 gastric cancer cases. The P-values represent the probabilities of USP14 mRNA levels between variable subgroups, as determined using the paired t-test (P = 0.0008). (D) Kaplan-Meier representation of the overall survival between two groups of patients with high (red line) and low (black line) USP14 protein expression. All data shown were collected from three independent experiments (P < 0.0001).