Global 3D chromatin structure of NK-92 cells is largely unaffected by Vorinostat treatment. (A) Hi-C contact maps generated by Juicebox at 250 kb, 100 kb, and 50 kb resolutions. (B) Distributions of first eigenvector values across the entirety of chromosome 1. (C) Saddle plots of compartmentalization strength with and without Vorinostat treatment. (D) Number of TAD boundaries obtained with Hi-C data. (E) Genome-wide averaged insulation scores plotted against distance around insulation center at TAD boundaries of control NK-92 cells. (F) Heatmaps show the average observed/expected Hi-C interactions in the TAD regions. (G) Distribution of regulatory elements at the anchors of H3K27ac HiChIP loops. (H) Volcano plot showing significant Vorinostat-induced changes in H3K27ac HiChIP loop strength. The number of loops exhibiting > 1.5-fold increases in control (orange) or Vorinostat (purple) with an FDR < 0.05 has been indicated.
