Genome-wide changes in gene expression and chromatin state of NK-92 in response to Vorinostat treatment. (A) Viability of NK-92 cells measured 6, 24, and 48 hours after treatment with various concentrations of Vorinostat (n = 3 per group). (B) Representative flow cytometric plots of the frequency of live cells measured 24 hours after treatment with 1 μM Vorinostat (n = 2 per group). (C) Scheme of NK-92 cells cultured with and without 1 μM Vorinostat for 24 hours. (D) RNA-seq MA plot of control versus Vorinostat. The number of genes exhibiting > 1.3-fold increases in control (orange) or Vorinostat (purple) with a false discovery rate (FDR) < 0.05 are indicated. (E) Gene ontology terms associated with upregulated (left) or downregulated (right) genes by Vorinostat treatment. (F) Immunoblot assay for H3K27ac levels in total cell lysates. (G) Volcano plot showing significant Vorinostat-induced changes on the H3K27ac level. The number of peaks exhibiting > 1.5-fold increases in control (orange) or Vorinostat (purple) with an FDR < 0.05 are indicated. (H) ATAC-seq scatter plot of control versus Vorinostat. The number of peaks exhibiting > 1.5-fold increases in control (orange) or Vorinostat (purple) with an FDR < 0.05 are indicated. (I) Snapshot of RNA-seq, ChIP-seq, and ATAC-seq signal tracks in the representative genomic region.
