GstO1 inhibition and reduced stability by C1-27. (A) 4-NPG reduction activity of GstO1 after incubation with the indicated concentrations of C1-27. The solid line fitting the data determined the IC50 = 220 ± 16 nM for C1-27. (B) E-GSH labeling of mGstO1 after incubation of 10 nM mGstO1 with the indicated increasing concentrations of C1-27 in the graph. mGstO1-SG-E was detected by 12% SDS-PAGE (lower) under UV-light (upper), and quantitatively analyzed by densitometry normalizing amount of loaded proteins (lower). (C, D) The thermal stability of GstO1 was determined by DSF and ITD at 37°C, respectively.
