Protein deglutathionylation by GstO1. (A) Fluorescence of free E-GSH released from E-GSH-labeled proteins following incubation with the indicated enzymes. (B) Western blot analysis for Pro-SG in reactions of (A) after labeling with biotin-NEM. (C) Western blot analysis for GstO1 and Pro-SG at the indicated incubation times after differentiation induction. (D) Western blot analysis for GstO1 and Pro-SG at 6 h and 24 h post-induction with 7 μM C1-27.