Direct interaction between netrin-1 and α-synuclein. (A) Real time binding assay with SPR Biacore X100 using the CM5 chip as a binding ligand. In vitro test was conducted with purified hnetrin-1 and hα-synuclein recombinant proteins. (B-D) Predictive three-dimensional model of direct interaction structures between netrin-1 (PDB ID: AF-O95631-F1) and α-synuclein (PDB ID: AF-P37840-F1) by ClusPro 2.0 and PyMOL 2.5 software analysis. Balanced model, electrostatic-favored model, and van der Waals force-favored model showed the polar contacts between netrin-1 and α-synuclein.