Knockdown of karyopherin-α3 (KPNA3-KD) inhibits cell migration and invasion through the transcriptional regulation of HAS2 and CDH1 in MDA-MB-231 cells. (A) Volcano plot indicating the general scattering of the transcripts identified by RNA sequencing analysis according to log2 (FC) and −log10 (P-value). Red-colored dots indicate genes filtered with absolute FC > 1.5 and −log10 > 20. Green-colored dots represent genes filtered with absolute FC > 1.5 and P < 0.05. Gray-colored dots indicate genes filtered with absolute FC < 1.5. (B) Venn diagram showing the overlap of genes downregulated in transcriptome profiles, genes upregulated in the EMT-core gene list, and genes upregulated in triple-negative breast cancer cells. (C) Expression level of HAS2 mRNA by RT-qPCR. Data are shown as the mean ± standard deviation (SD) of three independent experiments. (D) Expression level of HAS2 protein in KPNA3-KD-231 cells for 48 h by western blot analysis. (E) Expression levels of FLAG, E-cadherin, and KPNA3 proteins in KPNA3-KD-231 cells co-transfected with or without 3× FLAG-HAS2. (F) Cell images of migrated or invaded cells under the same conditions as those in (D) (left panel).
