The level of p300 during liver fibrosis. (A) Schematic showing the timeline of animal experiments for the CD-HFD model. (B) Representative photomicrographs of Sirius red staining, MTS, and immunohistochemistry (IHC) analysis of p300 in liver tissues from mice on ND and 1, 2, 4, 6, and 8 weeks after CD-HFD. (C) Quantification data of histological staining images (n = 5) by ImageJ software. Data are represented as mean ± SEM, *P < 0.05, ***P < 0.001, and ****P < 0.0001 by ordinary one-way ANOVA test. (D) The mRNA expression level of p300 in the liver of mice after 4 weeks of ND and CD-HFD (n = 5). ns = not significant. (E) Co-immunofluorescence of p300 and αSMA in mouse liver tissue at 8 weeks of ND and CD-HFD. (F) Western blot analysis of p300, αSMA, and Col1a in CD-HFD-induced mouse liver tissue. β-actin was used as the sample loading control.