Involvement of α-kleisin factor in the maintenance of genome integrity of ESCs. (A) Decrease in cell viability under REC8 or RAD21 depletion and DNA damage. (B) Cell viability analysis in cohesin-expressed ESCs with or without DNA damage by using HU. The apoptotic cells and live cells were analyzed by cell staining with PI and TO. (C, D) Quantification of the proportion of live cells and apoptotic cells. The cell survival rate was determined by flow cytometry and quantified with the BD Accuri C6 software. (E) Venn diagram illustrating the upregulated and downregulated genes when REC8 or RAD21 was depleted in ESCs. The red and blue numbers indicate the upregulated and downregulated genes in REC8- or RAD21-depleted ESCs relative to the siControl-treated ESCs. The black numbers represent the genes with the same RNA expression level in REC8- and RAD21-depleted ESCs and siControl-treated ESCs. The data were adjusted with P ≤ 0.05. (F) Heatmap of the expression of DNA repair genes relative to the siControl group. Red indicates upregulation, blue indicates downregulation, and white indicates the same expression. (G) Gene set enrichment analysis (GSEA) score of REC8- or RAD21-depleted ESCs for downregulated DNA repair genes. GSEA was performed through two independent experiments.