Astrocytic mitochondria enhance survival of DA neurons following 6-OHDA toxicity. (A) Schematic illustration of experimental timeline. (B, C) After performing neurotoxic insult with 20 μM 6-OHDA for 18 h to Nurr1-Mash1-overexpressing rat DA neurons, cells were treated with isolated astrocytic mitochondria twice. Immunofluorescence staining (B) and quantification of TH-positive neurons (C) are shown. Error bars, standard error (S.E.). **P < 0.01. Scale bar, 20 μm. DA, dopamine; 6-OHDA, 6-hydroxydopamine; TH, tyrosine hydroxylase.
