Effect of mitochondrial transplantation in an MCAO model. (A) Schematic illustration of experimental timeline. (B) Astrocyte-derived MitoTracker-labeled mitochondria (red) in the injected area (48 h). (C) Immunofluorescence images of TUJ1 expression and localization of injected MitoTracker-labeled mitochondria (red). White square highlights colocalization of MitoTracker and TUJ1 expression. (D) Typical TTC staining results. (E) Quantitation of infarct volumes. Error bars, standard error (S.E.). *P < 0.05. Scale bar, 200 μm. TUJ1, class III beta-tubulin; TTC, 2,3,5-triphenyltetrazolium chloride.
