The RRM domain of SRSF9 is required for HIV-1 inhibition. The schematic diagram of SRSF9 truncates (A). HEK293T cells were co-transfected with 1 μg of pNL4-3GFP with 250 ng of WT or 250 ng, 500 ng, or 1 ug of each truncate of SRSF9. Then, 24 h post-transfection, the cell supernatants were evaluated by Western blot (upper) and p24 ELISA (lower) (B). MT-4 cells were transduced with the same volume of supernatants for three days, and the GFP levels were analyzed by fluorescent microscopy (left) and FACS analysis (right) (C). The asterisks indicate a significant difference from control, analyzed by one-way ANOVA (*P < 0.05, **P < 0.005, and ***P < 0.001).