SRSF9 overexpression inhibits HIV-1 production. HEK293T cells were co-transfected with 1 ug pNL4-3GFP and 125 ng, 250 ng, 500 ng, or 1 μg flag-tagged SRSF9 expression vectors. As the control, a flag-tagged empty vector was used to equal the total amount of transfected DNA. After 24 h, virus production levels were measured by p24 enzyme-linked immunosorbent assay (p24 ELISA) in cell supernatants (A). The viral protein expression levels in cell lysates and supernatants were verified by Western blot (B). MT-4 cells were infected with the same volume of virus-containing culture supernatants. After 72 h, GFP levels were observed using a fluorescent microscope (C) and quantified by FACS analysis (D). The asterisks indicate a significant difference from control, analyzed by one-way ANOVA (*P < 0.05, **P < 0.005, and ***P < 0.001).
