Survival and histological analyses of glioblastoma-bearing mice after spRRV-TK gene therapy. (A) Survival of xenograft mice after spRRV-TK gene therapy. U-87 MG cells and viral vectors were stereotactically injected into the striata of BALB/c nude mice (n = 34). Survival curves were prepared using the Kaplan-Meier method. P < 0.0001. (B) Brain sections from the control group (spRRV-TK/PBS, 24 days after tumor establishment) and the GCV-treated group (spRRV-TK/GCV, 150 days after tumor establishment) were histologically analyzed by H&E staining.