spRRV provides more efficient transgene delivery to human glioblastoma cells than sRRV. (A, B) The replication kinetics of sRRV-FL (sRRVgp-RFP + sRRVe-GFP) and spRRV-FL (sRRVgp-RFP + spRRVe-GFP). U-87 MG cells transduced with sRRV-FL or spRRV-FL. (B) The percentages of sRRV-FL- and spRRV-FL-transduced cells that expressed GFP or RFP. (C) In vivo spread of sRRV-FL and spRRV-FL vectors. Each semi-RRV-FL vector combination was injected into the U-87 MG tumor xenograft (see Materials and Methods for details). Merged images for RFP and GFP expression patterns are shown for sRRV-FL and spRRV-FL transduced gliomas (lower panel). The same brain sections were then subjected to H&E staining (upper panel). (D) FACS analysis of viral vector spread. Intratumoral tissue was also collected from three mice 14 days after virus injection and analyzed by flow cytometry. Data are represented as mean ± SEM.
