(A) AACT in the plasma of healthy donors and vivax malaria patients detected by chemiluminescence (Blot #1): H numbered lanes are healthy group (1-5) and P numbered lanes (1-5) are patients. (B) Expression level of AACT in specimens was measured by comparing the band intensities of malaria-patient samples with those of healthy donors. Data in Ave. lanes are presented as means ± standard errors of five independent lanes. AACT expression differed significantly between plasma from healthy donors (#H1-#H5) and plasma from donors with vivax malaria (#P1-#P5; P < 0.01). (C) Expression level of AACT in specimens was measured by comparing the band intensities of each patient sample with that of the healthy donors (Blot #1-#20). (D) P < 0.01, compared with healthy donor (#H1-#H100) versus vivax malaria patients (#P1-#P100).