KCNK3 and BMPR2 expression is simultaneously reduced in PASMCs of rats with PAH. (A) Levels of KCNK3 and BMPR2 proteins in lung tissues of rats with MCT-induced PAH and normal controls were quantified using western blotting assay and expressed relative to levels in normal controls. Data represent mean ± SEM (*P < 0.05, **P < 0.01, n = 3 rats per group). (B) Representative immunofluorescence images of KCNK3 (green) and BMPR2 (red) in the small pulmonary arterioles (white dotted lined circles) of normal rats or rats with PAH. Nuclei were stained with DAPI (blue). Negative controls (NC) were treated with non-relevant primary IgGs. Scale bars = 20 μm.