Effects of Wnt activator on lengths of human hair follicles in a hyperglycemia model. Human hair follicles were cultured in control or high glucose (100 mM) medium with 1 μM KY19382 or 150 μM MNX for 2 or 6 days. (A, B) Hair shaft elongation of human follicles was measured for 6 days. (C) IHC analysis of human hair follicles for β-catenin and Ki67 for 2 days. (D, E) Quantitative measurements for nuclear and total β-catenin in human hair follicle cells. Scale bars: 100 μm for A, C. n = 10-12 follicles per group for B and n = 15 per group for D, E. Values are expressed as means ± SEM. *P < 0.05, ***P < 0.0005, NS, not significant.
