Influences of Wnt activator on WIHN in HFD and STZ-induced diabetic mice. Mouse wounds were treated with 1.5 mM KY19382 or 150 mM MNX for 21 or 35 days. (A) ALP staining to identify newly formed follicles of mice treated with each compound for 35 days. Dashed lines mean neogenic hair follicles. (B) Quantitative evaluation of ALP-positive newly formed follicles. (C) H&E staining to identify neogenic hair follicles in mice treated with each compound for 21 days and the relative number of neogenic hair follicles. Arrows indicate neogenic hair follicles. (D) IHC staining for fgf 9, keratin 17, β-catenin, and Ki67. Dashed lines indicate a boundary between epidermal and dermal regions. (E, F) Quantitative calculations for fluorescence intensities of total and nuclear β-catenin in neogenic hair follicle cells. (G) X-gal staining of mouse wounds from Axin2lacZ/+ mice. Scale bars: 100 μm for C and D. n = 5 mice per group for B, n = 12 per group for E, F. Values are expressed as means ± SEM. *P < 0.05, ***P < 0.0005, NS, not significant.