Effects of Wnt activator on hair cycle in HFD and STZ-induced diabetic mice. After plucking, mouse dorsal skins were treated with 1.5 mM KY19382 or 150 mM MNX for 5, 7 or 9 days. (A) Gross image showing the color of mouse backs at 9 days after plucking. (B) H&E staining at 5 day after treatment. (C) Quantitative calculations of each hair cycle stage. (D) IHC staining for keratin 15, β-catenin and Ki67 of dorsal skins at 5 days after treatment. (E, F) Quantitative evaluations for fluorescence intensities of total and nuclear β-catenin in bulge stem cells. (G) ALP staining of dorsal skins at 7 days. Scale bars: 100 μm for B, D, and G. n = 10 mice per group for C, n = 12 per group for E and F. Values are expressed as means ± SEM. **P < 0.005, ***P < 0.0005, NS, not significant.