Alteration of hair growth in HFD and STZ-induced diabetic mice. (A-D) Mouse dorsal skins were harvested at 5 or 7 days after depilation. (A) H&E staining of mouse dorsal skins at 5 day after depilation. (B) Quantitative analysis of each hair cycle stage. (C) IHC staining for keratin 15, Ki67, and β-catenin. (D) ALP staining at 7 days after depilation. (E-H) Mouse wounds were harvested at 21 or 35 days after wounding. (E) ALP staining to determine newly formed follicles at 35 days after wounding. (F) Quantitative calculation of neogenic hair follicles. (G) H&E staining showed neogenic hair follicles at 21 days after wounding. (H) IHC staining for keratin 17, fgf 9, β-catenin, and Ki67 in wound regions. Scale bars: 100 μm for A, C, D, G, and H. n = 5 mice per group for B and F. Values are expressed as means ± SEM. *P < 0.05.