MS-HRM assay for blood samples from 80 healthy people and 113 HCC patients. (A) Melting peak plot. The color codes are as follows: negative control, black; positive control, cyan; healthy people, green; and HCC patients, red. (B) Normalized melting curve plot. (C) Diagnostic performance of the MS-HRM assay and AFP test. (D) Scatter plots of AFP and AUMC values for tumor stage (early-stage: BCLC 0, A (yellow dot); late-stage: BCLC B, C, D (red dot)). AFP values were converted to log20. The gray dotted line on the y-axis represents the 95th percentile (7.325) of AUMC values of healthy people, and the gray dotted line on the x-axis sets indicates the tumor cut-off point where log20 (AFP) is 1.