MS-HRM assay of HCC tumor and paired normal tissue samples. (A) Melting peak plot of NAT and tumor tissue samples in the indicated temperature range. The color codes are as follows: negative control, black; positive control, cyan; NAT, blue; and tumor tissue, orange. (B) Normalized melting curve plot of NAT and tumor tissue samples. (C) Scatter plots for Cq and AUMC of each tissue sample. The gray dotted line indicates the 99th percentile of AUMC values of NATs. (D) Accuracy of the MS-HRM assay. Sensitivity and specificity are based on a cut-off set to the 99th percentile of AUMC values (7.463) of NATs.
