Methylation of cg22538054 in normal and tumor tissues. (A) The b-value of cg22538054 in public data collected from TCGA, GEO, and KNIH. WB; whole blood, LIHC; liver hepatocellular carcinoma. ****P < 0.0001. (B) Results of pyrosequencing analysis of eight CpG sites including cg22538054 at the USP44 promoter. The sequence of the USP44 promoter region, with CpG sites labeled “#number”, is shown at the top. Boxplot shows the cytosine frequencies of normal (gray) and tumor (black) tissues at CpG sites corresponding to the “#number” label shown above. ****P < 0.0001.