Combination of sorafenib with MG149 induces synergistic liver cancer cell death. (A) Cotreatment with sorafenib and MG149 facilitates cell death in all HCC cell lines. Representative images were acquired after 72 hrs of incubation with DMSO (0.1%) or drugs. Scale bars denote 300 μm. (B) Cell confluence under the indicated treatment for 72 hrs was determined by live-cell imaging analysis (n = 3). (C) Apoptotic cell death was analyzed by flow cytometry. Representative data are displayed. (D) The percentage of cells positively labeled with annexin-V was quantified from three independent FACS analyses (n = 3). ****P < 0.0001.
