Synergistic effect of sorafenib and MG149 on liver cancer cell proliferation. (A) Huh7 cells were treated with various concentrations of sorafenib for 72 hrs to determine the IC50 value for sorafenib. (B) A total of 93 small molecules in the focused chemical library were ordered by ‘Combination score’. (C) Cotreatment of sorafenib and MG149 in HCC cells decreased cell viability in a dose-dependent manner. (D) Synergism between sorafenib and MG149 was confirmed in all HCC cell lines. The CI value was calculated using CompuSyn software (n = 3) (28). *P < 0.05, **P < 0.01, ****P < 0.0001, ns, not statistically significant.