No difference was observed in CL 316,243-induced β3 adrenergic stimulation between the two genotypes. (A) Schematic diagram of CL injection experiment. (B) Body weight gain graph, (C) fat composition, (D) lean composition. (E) WAT weight differences normalized by total body weight, and (F) absolute fat weight differences of Saline injected WT, Saline injected KO, CL injected WT, and CL injected KO mice. (G) Relative mRNA expression of browning markers and (H) UCP1 protein expression in iWAT was normalized to the levels of α-actin. (I) H&E staining of eWAT, iWAT, and BAT. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
