An epidydimal adipose tissue macrophage is reduced in HFD treated KO mice. (A) F4/80 IHC staining of crown-like structure (CLS) formation in eWAT. (B) Number of CLSs was analyzed. (C) Relative mRNA expression of pro-inflammatory markers (M1 polarization). (D) Relative mRNA expression of anti-inflammatory markers (M2 polarization). (E) CCl2 mRNA expression. (F) Relative mRNA expression of apoptosis markers. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
