Tollip decreases the interaction of PINK1 with TOM20 and parkin after treatment with CCCP. (A-C) HEK293 cells were transfected for 48 h with a plasmid encoding Myc-PINK1 or Xpress-Tollip (A, B), Myc-PINK1, or Tollip-siRNA, alone or in combination (C). Cells were then treated for 4 h with 10 μM CCCP. (D) Tollip+/+ and Tollip−/− MEFs were transfected for 48 h with a plasmid encoding Myc-PINK1 and treated for an additional 4 h with 10 μM CCCP. All data in the graphs are presented as the mean ± SEM of three independent experiments (**P ≤ 0.01; A-C, *P ≤ 0.05; D).