Various cellular functions of H2S in in the liver. Three major enzymes responsible for H2S production are CBS, CSE, and MPST. L-cysteine is the major substrate for H2S production. H2S–mediated signaling varieties from protein modification by sulfidation to affecting a broad range of physiological processes, including regulation of mitochondrial biogenesis, glucose metabolism, oxidative stress, inflammation, fatty acid oxidation and crosstalk with other signaling molecules. CBS: cystathionine β-synthase; CSE: cystathionine γ-lyase; MPST: 3-mercaptopyruvate sulfur transferase.