Effects of 2-MCA on Inducing Autophagy Flux in RAW264.7 (A) RAW264.7 cells containing luciferase-LC3 were pre-treated with or without 2-MCA (50 μM) for 4 h. After LPS (1 μg/ml) or BafA1 (40 nM) treatment for 8 h, autophagy flux was measured using luciferase assay. (B, C) RAW264.7 cells were pre-treated with or without 2-MCA (50 μM) for 4 h. After LPS (1 μg/ml), BafA1 (40 nM), or NH4Cl (10 mM) treatment for 8 h, the expression levels of Tnf and Nos2 were analyzed using qRT-PCR assay. The results from three independent experiments are presented as means ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001.
