Effect of 2-MCA on the expression of NRF2 and ATF3 in RAW264.7. (A) RAW264.7 cells were pre-treated with or without 2-MCA (50 μM) for 4 h. After LPS (1 μg/ml) treatment within the indicated time, the kinetic expression levels of NRF2 and ATF3 were analyzed using immunoblot assay. (B) RAW264.7 cells were pre-treated with or without 2-MCA (50 μM) for 4 h. After LPS (1 μg/ml) treatment within the indicated time, cells were fractionated into cyto-sol/membrane and nucleus fractions. The nucleus translocations of p65, NRF2, and ATF3 were assessed. (C) RAW264.7 cells, whether pre-treated with or without 2-MCA (50 μM) for 4 h, were stimulated with LPS (1 μg/ml) as indicated. The expression levels of NRF2, ATF3, HO-1, and NOS2 were evaluated using immunoblot assay. (D) RAW264.7 cells, whether pre-incubated with or without 2-MCA as indicated for 4 h, were stimulated with LPS (1 μg/ml) for 4 h. The expression levels of NRF2, ATF3, HO-1, and NOS2 were assessed using immunoblot assay.
