Anti-inflammatory effects of 2-MCA in LPS-stimulated macrophages. (A) RAW264.7 cells (left) and BMMs (right) were incubated with 2-MCA as indicated for 24 h. The absorbance produced by MTS was measured at 490 nm. The relative cell viability is shown as the mean ± SD (n = 3, with triplicates in each experiment). (B) RAW264.7 cells (left) and BMMs (right) were pre-incubated with 2-MCA as indicated for 4 h, followed by incubation with or without LPS (1 μg/ml) for 24 h. The secreted TNF-α in the culture media was analyzed by ELISA; *P < 0.05, **P < 0.01. (C) RAW264.7 (left) and BMMs (right) were incubated with 2-MCA as indicated for 4 h, followed by incubation with or without LPS (1 μg/ml) for 24 h. The released NO was evaluated by Griess Reagent-based analysis; *P < 0.05, **P < 0.01, ***P < 0.001.
