Ahnak depletion influences the TGF-β1/Smad signaling pathway during liver regeneration after partial hepatectomy (PH). (A) Ahnak mRNA expression in wild type (WT) mice at different time points after PH (n = 3). (B) HGF mRNA expression in wild-type (WT) mice at different time points after PH (n = 3). (C) TGF-β1 mRNA expression in Ahnak knockout (KO) and WT mice at the indicated time points after PH (n = 3). (D) Representative western blots of SMAD2, phospho-SMAD2, SMAD3, and phospho-SMAD3 at the indicated time points. *P < 0.05; Error bars represent the mean ± SD.
