2-Undecanone decreases neutrophil cell survival under LPS stimulation. (A, B) Mouse neutrophils were stimulated with LPS (1 μg/ml) and vehicle or 2-undecanone (200 μM) for 2 h. (A) The levels of p-ERK, t-ERK, p-Akt, t-Akt, and β-actin, (B) cleaved caspase-3, Bcl-2, and β-actin were measured using Western blot analysis. (C) Apoptosis of neutrophils was determined by anti-Annexin V antibody and PI staining. Neutrophils were stimulated with or without LPS (1 μg/ml) and vehicle or 2-undecanone (200 μM) for 2 h and 12 h. The data are representative of three independent experiments. Data are expressed as the mean ± SEM *P < 0.05, **P < 0.01, ***P < 0.001 by two-way ANOVA (C bottom).
