CK2α strongly binds to α-tubulin acetyltransferase 1 (α-TAT1) on the soft matrix. (A, B) Co-immunoprecipitation (Co-IP) assay was performed to analyze the interaction between Flag-CK2α and green fluorescent protein (GFP)-α-TAT1 in HEK293T cells. (C) Co-IP assay was performed to analyze the interaction between Flag-CK2α and GFP-α-TAT1 with or without blebbistatin in WT MEFs. (D) Cellular localization of Flag-CK2α and GFP-α-TAT1 in MEFs with or without blebbistatin was observed via immunocytochemistry using anti-FLAG antibodies. White arrows indicate the colocalization of Flag-CK2α and GFP-α-TAT1. Scale bars: 25 μm and 5 μm (cropped image). Graphs show the fluorescence intensities of Flag-CK2α (red) and GFP-α-TAT1 (green).