Spatial distribution of histone modifications detected using plasmonic nanoparticles conjugated to histone antibodies. (A) Schematic diagram of nanoprobe-conjugated H3K9me3 antibody and of the specific arrangements of histone octamers in young growing cells (upper) and oncogene-induced senescent (OIS) cells (lower). (B) Representative dark-field scattering images of plasmonic nanoprobes targeting heterochromatin histone markers in a young growing cell (upper) and a senescent (lower) cell. Scale bar, 10 μm. (C) Relationship between the spectral peak (λmax) of simulated scattering and the spatial arrangements of plasmonic nanoprobes. This diagram was reproduced with permission from the cited reference.
