JAK2 is downregulated during neuronal differentiation. (A) Expression levels of JAK2 and phosphorylated (p)-JAK2 in J1 embryonic stem cells (ESCs) at various time points during differentiation culture as analyzed by immunoblotting. (B) Immunofluorescence analysis of J1 ESCs and differentiated neurons. Phosphorylated JAK2 was stained green.