Experimental procedure; body weight (BW, g), GAS skeletal muscle tissue weight (GAS, g), and GAS normalized by BW among CON (n = 10), 0.1 mg (n = 6), and 0.5 mg (n =10) groups. (A) Experimental procedure. (B) Changes in BW over 15-days (time) for each group. (C) Differences in GAS of CON (n = 8) and 0.1 mg (n = 5) groups as compared to the 0.5 mg (n = 9) group. (D) Levels of normalization of GAS by BW in CON (n = 6), 0.1 mg (n = 4) and 0.5 mg (n = 7) groups. Values were determined by densitometric quantification and are shown as the mean ± standard error of the mean (SEM). Outliers in each group were excluded. CON, saline control; 0.1 mg, 0.1 mg/kg BW of cisplatin administered in skeletal muscle; 0.5 mg, 0.5 mg/kg BW of cisplatin administered in skeletal muscle. Statistical methods used were one and two-way repeated ANOVA with the factor time for BW and one-way ANOVA with Tukey’s post-hoc test for GAS and normalization of GAS and BW analyses. Units are arbitrary. ***P < 0.001 vs Time and Group in (B) ***P < 0.001 vs CON and ###P < 0.001 vs 0.1 mg in (C).